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Product Description
Rat GCP2 (Granulocyte Chemotactic Protein 2) ELISA Kit | ELK6259 | ELK Biotechnology
Alternative Names: CXCL6; SCYB6; GCP2; CKA-3; Chemokine (C-X-C-Motif) ligand 6; Small Inducible Cytokine Subfamily B (Cys-X-Cys) Member 6; Chemokine alpha 3; Small-inducible cytokine B6
Species: Rat
Assay Type: Sandwich
Sensitivity: 14.8 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Lenght: 3.5h
Research Area: Cytokine;Infection immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Granulocyte Chemotactic Protein 2 (GCP2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Granulocyte Chemotactic Protein 2 (GCP2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Granulocyte Chemotactic Protein 2 (GCP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Granulocyte Chemotactic Protein 2 (GCP2) in the samples is then determined by comparing the OD of the samples to the standard curve.
