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Product Description
Rat HCII (Heparin Cofactor II) ELISA Kit | ELK2889 | ELK Biotechnology
Alternative Names: SERPIND1; HC2; HCF2; HC II; HLS2; LS2; Serpin D1; Serpin Peptidase Inhibitor Clade D Member 1; Protease inhibitor leuserpin-2
Species: Rat
Assay Type: Sandwich
Sensitivity: 3.3 ng/mL
Standard: 500 ng/mL
Detection Range: 7.82-500 ng/mL
Sample Type: Plasma.
Assay Lenght: 3.5h
Research Area: Hematology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heparin Cofactor II (HCII) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Heparin Cofactor II (HCII) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heparin Cofactor II (HCII), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heparin Cofactor II (HCII) in the samples is then determined by comparing the OD of the samples to the standard curve.
