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Product Description
Rat HSPb6 (Heat Shock Protein Beta 6) ELISA Kit | ELK4263 | ELK Biotechnology
Alternative Names: HSP20; Heat Shock Protein 20 kD; Heat Shock Protein B6, Alpha Crystallin Related; Heat shock 20 kDa-like protein p20
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.068 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Infection immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein Beta 6 (HSPb6) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Heat Shock Protein Beta 6 (HSPb6) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein Beta 6 (HSPb6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock Protein Beta 6 (HSPb6) in the samples is then determined by comparing the OD of the samples to the standard curve.
