Euro
USD
British Pound
NULL
Product Description
Rat IDE (Insulin Degrading Enzyme) ELISA Kit | ELK6425 | ELK Biotechnology
Alternative Names: Insulysin; Insulin Protease; Abeta-degrading protease; Insulinase
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.112 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase;Metabolic pathway;Endocrinology;Cardiovascular biology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Degrading Enzyme (IDE) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Insulin Degrading Enzyme (IDE) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Degrading Enzyme (IDE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Insulin Degrading Enzyme (IDE) in the samples is then determined by comparing the OD of the samples to the standard curve.
