Euro
USD
British Pound
NULL
Product Description
Rat IgD (Immunoglobulin D) ELISA Kit | ELK7625 | ELK Biotechnology
Alternative Names: IGHD; Constant Region Of Heavy Chain Of Immunoglobulin Delta
Species: Rat
Assay Type: Competitive Inhibition
Sensitivity: 53.7 pg/mL
Standard: 10000 pg/mL
Detection Range: 156.25-10000 pg/mL
Sample Type: Serum, plasma and other biological fluids
Assay Lenght: 2h
Research Area: Infection immunity;Immune molecule
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Immunoglobulin D (IgD) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Immunoglobulin D (IgD) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Immunoglobulin D (IgD) in the samples is then determined by comparing the OD of the samples to the standard curve.
