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Product Description
Rat IRS2 (Insulin Receptor Substrate 2) ELISA Kit | ELK7043 | ELK Biotechnology
Alternative Names: Insulin receptor substrate 2; IRS 2; IRS-2; Irs2
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.33 ng/mL
Standard: 50 ng/mL
Detection Range: 0.79-50 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Endocrinology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Receptor Substrate 2 (IRS2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Insulin Receptor Substrate 2 (IRS2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Receptor Substrate 2 (IRS2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Insulin Receptor Substrate 2 (IRS2) in the samples is then determined by comparing the OD of the samples to the standard curve.
