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Product Description
Rat LGALS3BP (Lectin Galactoside Binding, Soluble 3 Binding Protein) ELISA Kit | ELK7536 | ELK Biotechnology
Alternative Names: MAC-2-BP; LGALS3-BP; Galectin-3-Binding Protein; L3 Antigen; Mac-2-Binding Protein; Tumor-associated antigen 90K; Serum Protein 90K; Basement membrane autoantigen p105
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.64 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Lenght: 3.5h
Research Area: Tumor immunity;Infection immunity;Immunodeficiency
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lectin Galactoside Binding, Soluble 3 Binding Protein (LGALS3BP) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Lectin Galactoside Binding, Soluble 3 Binding Protein (LGALS3BP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lectin Galactoside Binding, Soluble 3 Binding Protein (LGALS3BP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lectin Galactoside Binding, Soluble 3 Binding Protein (LGALS3BP) in the samples is then determined by comparing the OD of the samples to the standard curve.
