Euro
USD
British Pound
NULL
Product Description
Rat LIPG (Lipase, Endothelial) ELISA Kit | ELK1434 | ELK Biotechnology
Alternative Names: EL; EDL; Endothelial Lipase; Endothelial cell-derived lipase
Species: Rat
Assay Type: Sandwich
Sensitivity: 1.15 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase;Metabolic pathway
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipase, Endothelial (LIPG) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Lipase, Endothelial (LIPG) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipase, Endothelial (LIPG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lipase, Endothelial (LIPG) in the samples is then determined by comparing the OD of the samples to the standard curve.
