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Product Description
Rat LRG1 (Leucine Rich Alpha-2-Glycoprotein 1) ELISA Kit | ELK5706 | ELK Biotechnology
Alternative Names: A2GL ; HMFT1766 ; Leucine rich alpha 2 glycoprotein ; Leucine-rich alpha-2-glycoprotein 1 ; LRG ; LRG1
Species: Rat
Assay Type: Sandwich
Sensitivity: 1.44 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Lenght: 3.5h
Research Area: Signal transduction;Tumor immunity;Infection immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leucine Rich Alpha-2-Glycoprotein 1 (LRG1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Leucine Rich Alpha-2-Glycoprotein 1 (LRG1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leucine Rich Alpha-2-Glycoprotein 1 (LRG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Leucine Rich Alpha-2-Glycoprotein 1 (LRG1) in the samples is then determined by comparing the OD of the samples to the standard curve.
