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Product Description
Rat MAP1LC3B (Microtubule-associated proteins 1A/1B light chain 3B) ELISA Kit | ELK8900 | ELK Biotechnology
Alternative Names: LC3B; MAP1LC3B; MAP1ALC3; Microtubule-associated proteins 1A/1B light chain 3B; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.067 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Metabolic pathway
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B) in the samples is then determined by comparing the OD of the samples to the standard curve.
