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Product Description
Rat MGMT (O-6-Methylguanine DNA Methyltransferase) ELISA Kit | ELK7160 | ELK Biotechnology
Alternative Names: Methylated-DNA--Protein-Cysteine Methyltransferase; 6-O-methylguanine-DNA methyltransferase; Methylated-DNA--protein-cysteine methyltransferase
Species: Rat
Assay Type: Sandwich
Sensitivity: 27 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Signal transduction;Enzyme & Kinase;Tumor immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to O-6-Methylguanine DNA Methyltransferase (MGMT) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to O-6-Methylguanine DNA Methyltransferase (MGMT) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain O-6-Methylguanine DNA Methyltransferase (MGMT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of O-6-Methylguanine DNA Methyltransferase (MGMT) in the samples is then determined by comparing the OD of the samples to the standard curve.
