Euro
USD
British Pound
NULL
Product Description
Rat MIP1g (Macrophage Inflammatory Protein 1 Gamma) ELISA Kit | ELK7711 | ELK Biotechnology
Alternative Names: CCL9; CCL10; SCYB9; CCF18; MRP2; C-C motif chemokine 9; Macrophage inflammatory protein-related protein 2; Small-inducible cytokine A9
Species: Rat
Assay Type: Sandwich
Sensitivity: 7.2 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 3.5h
Research Area: Cytokine;Infection immunity;Human gene deletion
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Inflammatory Protein 1 Gamma (MIP1g) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Macrophage Inflammatory Protein 1 Gamma (MIP1g) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Macrophage Inflammatory Protein 1 Gamma (MIP1g), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Macrophage Inflammatory Protein 1 Gamma (MIP1g) in the samples is then determined by comparing the OD of the samples to the standard curve.
