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Product Description
Rat NT5E (5'-Nucleotidase, Ecto) ELISA Kit | ELK6905 | ELK Biotechnology
Alternative Names: CD73; NT5-E; E5NT; E5-NT; NTE; EN; ENT
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.261 ng/mL
Standard: 40 ng/mL
Detection Range: 0.63-40 ng/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Lenght: 3.5h
Research Area: Infection immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 5'-Nucleotidase, Ecto (NT5E) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5'-Nucleotidase, Ecto (NT5E) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 5'-Nucleotidase, Ecto (NT5E), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5'-Nucleotidase, Ecto (NT5E) in the samples is then determined by comparing the OD of the samples to the standard curve.
