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Product Description
Rat PROC (Protein C) ELISA Kit | ELK2837 | ELK Biotechnology
Alternative Names: PRO-C; VKDPC; PC; Vitamin K Dependent Protein C; Inactivator Of Coagulation Factors Va And VIIIa; Autoprothrombin IIA; Blood coagulation factor XIV; Activation peptide
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.58 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: Plasma.
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase;Hematology;Cardiovascular biology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein C (PROC) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Protein C (PROC) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein C (PROC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein C (PROC) in the samples is then determined by comparing the OD of the samples to the standard curve.
