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Product Description
Rat PTGS1 (Prostaglandin Endoperoxide Synthase 1) ELISA Kit | ELK1859 | ELK Biotechnology
Alternative Names: COX1; COX3; PCOX1; PGG/HS; PGHS1; PHS1; PTGHS; Cyclooxygenase 1; Prostaglandin H2 Synthase 1; Prostaglandin G/H Synthase And Cyclooxygenase
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.245 ng/mL
Standard: 40 ng/mL
Detection Range: 0.63-40 ng/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Lenght: 3.5h
Research Area: Signal transduction;Enzyme & Kinase;Tumor immunity;Infection immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prostaglandin Endoperoxide Synthase 1 (PTGS1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Prostaglandin Endoperoxide Synthase 1 (PTGS1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prostaglandin Endoperoxide Synthase 1 (PTGS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prostaglandin Endoperoxide Synthase 1 (PTGS1) in the samples is then determined by comparing the OD of the samples to the standard curve.
