Product Description
Rat SCGN (Secretagogin) ELISA Kit | ELK6364 | ELK Biotechnology
Alternative Names: SECRET; SEGN; CALBL; Calbindin-Like; Secretagogin, EF-Hand Calcium Binding Protein
Species: Rat
Assay Type: Competitive Inhibition
Sensitivity: 24.7 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Lenght: 2h
Research Area: Metabolic pathway
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Secretagogin (SCGN) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Secretagogin (SCGN) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Secretagogin (SCGN) in the samples is then determined by comparing the OD of the samples to the standard curve.