Euro
USD
British Pound
NULL
Product Description
Rat SQSTM1 (Sequestosome 1) ELISA Kit | ELK8901 | ELK Biotechnology
Alternative Names: OSIL; PDB3; ZIP3; p62; p62B; p60; A170; Paget Disease Of Bone 3; Oxidative Stress Induced Like; EBI3-associated protein of 60 kDa; Ubiquitin-binding protein p62
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.113 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Signal transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sequestosome 1 (SQSTM1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sequestosome 1 (SQSTM1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sequestosome 1 (SQSTM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sequestosome 1 (SQSTM1) in the samples is then determined by comparing the OD of the samples to the standard curve.
