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Product Description
Rat T4 (Thyroxine) ELISA Kit | ELK8716 | ELK Biotechnology
Alternative Names: 3, 5, 3', 5'-Tetraiodothyronine; L-Thyroxine; Levothyroxine
Species: Rat
Assay Type: Competitive Inhibition
Sensitivity: 1.42 ng/mL
Standard: 300 ng/mL
Detection Range: 4.69-300 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Lenght: 2h
Research Area: Endocrinology;Hormone metabolism
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Thyroxine (T4) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thyroxine (T4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thyroxine (T4) in the samples is then determined by comparing the OD of the samples to the standard curve.
