Euro
USD
British Pound
NULL
Product Description
Rat TGFbR1 (Transforming Growth Factor Beta Receptor I) ELISA Kit | ELK5317 | ELK Biotechnology
Alternative Names: ACVRLK4; AAT5; ALK5; SKR4; TGFR-1; Activin A Receptor Type II-Like Kinase 53kDa; Activin receptor-like kinase 5; Serine/threonine-protein kinase receptor R4
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.122 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Tumor immunity;Infection immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transforming Growth Factor Beta Receptor I (TGFbR1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Transforming Growth Factor Beta Receptor I (TGFbR1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transforming Growth Factor Beta Receptor I (TGFbR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transforming Growth Factor Beta Receptor I (TGFbR1) in the samples is then determined by comparing the OD of the samples to the standard curve.
