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Product Description
Rat TNNI2 (Troponin I Type 2, Fast Skeletal) ELISA Kit | ELK6239 | ELK Biotechnology
Alternative Names: AMCD2B; DA2B; FSSV; Troponin I Fast Twitch 2; Troponin I, fast-twitch isoform
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.65 ng/mL
Standard: 150 ng/mL
Detection Range: 2.35-150 ng/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Lenght: 3.5h
Research Area: Cardiovascular biology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Troponin I Type 2, Fast Skeletal (TNNI2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Troponin I Type 2, Fast Skeletal (TNNI2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Troponin I Type 2, Fast Skeletal (TNNI2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Troponin I Type 2, Fast Skeletal (TNNI2) in the samples is then determined by comparing the OD of the samples to the standard curve.
