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Product Description
Rat TOP1 (Topoisomerase I) ELISA Kit | ELK7553 | ELK Biotechnology
Alternative Names: TOPI; DNA Topoisomerase 1
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.056 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase;Developmental science
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Topoisomerase I (TOP1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Topoisomerase I (TOP1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Topoisomerase I (TOP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Topoisomerase I (TOP1) in the samples is then determined by comparing the OD of the samples to the standard curve.
