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Product Description
Fish SDHA (Succinate Dehydrogenase Complex Subunit A) ELISA Kit | ELK8515 | ELK Biotechnology
Alternative Names: SDH2; SDHF; FP; Flavoprotein; Flavoprotein subunit of complex II; Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial
Species: Fish
Assay Type: Sandwich
Sensitivity: 11.81 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Succinate Dehydrogenase Complex Subunit A (SDHA) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Succinate Dehydrogenase Complex Subunit A (SDHA) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Succinate Dehydrogenase Complex Subunit A (SDHA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Succinate Dehydrogenase Complex Subunit A (SDHA) in the samples is then determined by comparing the OD of the samples to the standard curve.
