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Product Description
General ECP (Escherichia coli Protein) ELISA Kit | ELK6654 | ELK Biotechnology
Alternative Names: ECP
Species: General
Assay Type: Sandwich
Sensitivity: 0.125 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Biological agents
Assay Lenght: 3.5h
Research Area: Gastroenterology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Escherichia coli Protein (ECP) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Escherichia coli Protein (ECP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Escherichia coli Protein (ECP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Escherichia coli Protein (ECP) in the samples is then determined by comparing the OD of the samples to the standard curve.
