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Product Description
Guinea pig VIP (Vasoactive Intestinal Peptide) ELISA Kit | ELK7054 | ELK Biotechnology
Alternative Names: PHM27; Intestinal peptide PHV-42; Peptide histidine valine 42; Peptide histidine methioninamide 27
Species: Guinea pig
Assay Type: Sandwich
Sensitivity: 2.52 pg/mL
Standard: 500 pg/mL
Detection Range: 7.82-500 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Lenght: 3.5h
Research Area: Endocrinology;Gastroenterology;Hormone metabolism
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vasoactive Intestinal Peptide (VIP) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Vasoactive Intestinal Peptide (VIP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vasoactive Intestinal Peptide (VIP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vasoactive Intestinal Peptide (VIP) in the samples is then determined by comparing the OD of the samples to the standard curve.
