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Product Description
Spinach Trx (Thioredoxin) ELISA Kit | ELK7909 | ELK Biotechnology
Alternative Names: TXN; ADF; SASP; TRDX; TRX1; ATL-derived factor; Surface-associated sulphydryl protein
Species: Spinach
Assay Type: Sandwich
Sensitivity: 0.124 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Plant tissue or cell culture supernates.
Assay Lenght: 3.5h
Research Area: Metabolic pathway;Developmental science
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thioredoxin (Trx) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thioredoxin (Trx) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thioredoxin (Trx), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thioredoxin (Trx) in the samples is then determined by comparing the OD of the samples to the standard curve.
