Euro
USD
British Pound
NULL
Product Description
Anti- PUM2 Antibody | FNab06955 | FineTest
Form: liquid
Purification: Immunogen affinity purified
Purity: ≥95% as determined by SDS-PAGE
Host: Rabbit
Clonality: polyclonal
Clone ID: N/A
Isotype: IgG
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Background: Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE) (, PubMed:21397187) . Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation (PubMed:22955276) . Also mediates deadenylation-independent repression by promoting accessibility of miRNAs (PubMed:18776931, PubMed:22345517) . Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3'-UTR and facilitating miRNA regulation (PubMed:22345517) . Plays a role in cytoplasmic sensing of viral infection (PubMed:25340845) . Represses a program of genes necessary to maintain genomic stability such as key mitotic, DNA repair and DNA replication factors. Its ability to repress those target mRNAs is regulated by the lncRNA NORAD (non-coding RNA activated by DNA damage) which, due to its high abundance and multitude of PUMILIO binding sites, is able to sequester a significant fraction of PUM1 and PUM2 in the cytoplasm (PubMed:26724866) . May regulate DCUN1D3 mRNA levels (PubMed:25349211) . May support proliferation and self-renewal of stem cells.
Immunogen: pumilio homolog 2 (Drosophila)
synonyms: KIAA0235, PUMH2
Molecular weight (observed) : 140 kDa
Reactivity Species: Human, Mouse, Rat
Tested Application: ELISA, WB, IHC, IF, IP
Recomended Dillution : WB: 1:500-1:2000
Research Area: Stem Cells, Immunology, Metabolism
