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Product Description
Rheumatoid Factor IgA ELISA kit | 55R-ORG522A | Fitzgerald
Activity Code: ACTIVE
Product Type: Research Kits
Product Subtype: Kits
Research Area: Cell Biology
Short Description: ELISA kit for the detection of Rheumatoid Factor IgA in the research laboratory
Immunogen: N/A
Host: N/A
Specificity: N/A
Cross Reactivity: N/A
Isotype: N/A
IClone: N/A
Species: N/A
Residues: N/A
Tag/conjugate: N/A
Protein Type: N/A
Expression System: N/A
Grade & Purity: N/A
Methode of Purification: N/A
Source: N/A
Concentration: N/A
Form & Buffer: N/A
Storage: Store at 2-8 deg C
Shipping Information: *Blue Ice*
Applications: ELISA
Bioactivity: N/A
Usage Recommendations: Optimal conditions to be determined by end-user
Assay Information: Fc fragments of highly purified human Immunoglobulin G are bound to microwells. Antibodies against this antigen, if present in diluted serum or plasma, bind to the respective antigen. Washing of the microwells removes unspecific serum and plasma components. Horseradish peroxidase (HRP) conjugated antihuman IgA immunologically detects the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyses to form a blue colour. The addition of an acid stops the reaction forming a yellow endproduct. The intensity of this yellow colour is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgA antibodies present in the original sample.
