Product Description
GSTM2 protein (His tag) | 80R-1490 | Fitzgerald
Activity Code: ACTIVE
Product Type: Proteins
Product Subtype: Conjugated Proteins
Research Area: Signal Transduction
Short Description: Purified recombinant Human GSTM2 protein
Immunogen: N/A
Host: N/A
Specificity: N/A
Cross Reactivity: N/A
Isotype: N/A
IClone: N/A
Species: Human
Residues: 1-218 amino acids: MGSSHHHHHH SSGLVPRGSH MPMTLGYWNI RGLAHSIRLL LEYTDSSYEE KKYTMGDAPD YDRSQWLNEK FKLGLDFPNL PYLIDGTHKI TQSNAILRYI ARKHNLCGES EKEQIREDIL ENQFMDSRMQ LAKLCYDPDF EKLKPEYLQA LPEMLKLYSQ FLGKQPWFLG DKITFVDFIA YDVLERNQVF EPSCLDAFPN LKDFISRFEG LEKISAYMKS SRFLPRPVFT KMAVWGNK
Tag/conjugate: His tag
Protein Type: Recombinant
Expression System: E.coli
Grade & Purity: > 95% pure
Methode of Purification: N/A
Source: N/A
Concentration: 0.5 mg/ml
Form & Buffer: Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol, 0.1M NaCl and 1mM DTT.
Storage: Store at 4 deg C for short term storage (1/2 weeks) . Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Shipping Information: *Blue Ice*
Applications: SDS-PAGE
Bioactivity: Specific activity is < 25 units/mg, and is defined as the amount of enzyme that conjugate 1.0 uM of 1-chloro-2, 4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25 DEG C.
Usage Recommendations: N/A
Assay Information: 1. Prepare a 1 ml reaction mix into a suitable container: The final concentrations are 97 mM potassium phosphate, 0.97 mM EDTA, 2.5 mM glutathione, reduced, 1.0 mM 1-chloro-2, 4-dinitrobenzene (CDNB), 3.2% (v/v) ethanol. 2. Equilibrate to 25 DEG C and monitor at A340nm until the value is constant using a spectrophotometer. 3. Add 50ul of recombinant GSTM2 protein with various concentrations (1ug, 2ug, 5ug) in 950ul reaction buffer. 4. Mix by inversion and record the increase at A340nm for 5 minutes.