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Product Description
Thioredoxin 1 protein (His tag) | 80R-1313 | Fitzgerald
Activity Code: ACTIVE
Product Type: Proteins
Product Subtype: Conjugated Proteins
Research Area: Proteases, Inhibitors, & Enzymes
Short Description: Purified recombinant E.coli Thioredoxin 1 protein
Immunogen: N/A
Host: N/A
Specificity: N/A
Cross Reactivity: N/A
Isotype: N/A
IClone: N/A
Species: E.coli
Residues: 2-109 amino acids: MHHHHHHMGS DKIIHLTDDS FDTDVLKADG AILVDFWAEW CGPCKMIAPI LDEIADEYQG KLTVAKLNID QNPGTAPKYG IRGIPTLLLF KNGEVAATKV GALSKGQLKE FLDANLAGS
Tag/conjugate: His tag
Protein Type: Recombinant
Expression System: E.coli
Grade & Purity: > 85% pure
Methode of Purification: N/A
Source: N/A
Concentration: 0.5 mg/ml
Form & Buffer: Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol and 1mM DTT.
Storage: Store at 4 deg C for short term storage (1/2 weeks) . Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Shipping Information: *Blue Ice*
Applications: SDS-PAGE
Bioactivity: Specific activity is 16~18 A650/min/mg and was obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.
Usage Recommendations: N/A
Assay Information: 1. Prepare a 1ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris, 63mM sodium phosphate, 1mM DTT, 0.1% (w/v) insulin, 2mM EDTA. 2. Add 2ug of recombinant E.coli thioredoxin into reaction cocktail and mix immediately. 3. Incubate at 25 DEG C for 20 minutes. 4. Record the increase in A650nm for 15 minutes.
