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Product Description
VE Cadherin antibody | 10R-V102b | Fitzgerald
Activity Code: ACTIVE
Product Type: Primary Antibodies
Product Subtype: Monoclonal Antibodies
Research Area: Nutrition & Metabolism
Short Description: Mouse monoclonal VE Cadherin antibody
Immunogen: VE Cadherin antibody was raised in Mouse using HUVEC cells as the immunogen.
Host: Mouse
Specificity: Human
Cross Reactivity: N/A
Isotype: IgG2a, kappa
IClone: BV6
Species: N/A
Residues: N/A
Tag/conjugate: N/A
Protein Type: N/A
Expression System: 293 cells
Grade & Purity: N/A
Methode of Purification: VE Cadherin antibody was purified using Protein G affinity chromatography.
Source: N/A
Concentration: 500 ug/ml
Form & Buffer: Supplied in liquid form in 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage: Stable for 1 year at 2-8 deg C from date of receipt.
Shipping Information: *Blue Ice*
Applications: FC, IHC-P, ICC, WB
Bioactivity: N/A
Usage Recommendations: ICC: 1:500; IHC-P: 1:10-1:20; User optimization required for FC and WB.
Assay Information: IHC: Buffered formalin fixation is recommended with a fixation period of < 12 hours. High heat antigen retrieval in citrate buffer is suggested. Antibody can also be used to label acetone-fixed cryostat sections or cells. FC: Use in the presence of Ca ++. PBS/2-5mM EDTA is recommended for cell detachment. WB: Non-reducing conditions may be needed, 2-5 mM Ca++ required in buffer.
