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Product Description
FKBP2 protein | 30R-1506 | Fitzgerald
Activity Code: ACTIVE
Product Type: Proteins
Product Subtype: Recombinant Proteins
Research Area: Cell Cycle & Cell Death
Short Description: Purified recombinant Human FKBP2 protein
Immunogen: N/A
Host: N/A
Specificity: N/A
Cross Reactivity: N/A
Isotype: N/A
IClone: N/A
Species: Human
Residues: 22-142 amino acids: MATGAEGKRK LQIGVKKRVD HCPIKSRKGD VLHMHYTGKL EDGTEFDSSL PQNQPFVFSL GTGQVIKGWD QGLLGMCEGE KRKLVIPSEL GYGERGAPPK IPGGATLVFE VELLKIERRT EL
Tag/conjugate: N/A
Protein Type: Recombinant
Expression System: E.coli
Grade & Purity: > 90% pure
Methode of Purification: N/A
Source: N/A
Concentration: 1 mg/ml
Form & Buffer: Supplied as a liquid in 20mM Tris buffer, pH 8.0, containing 10% glycerol and 1mM DTT.
Storage: Store at 4 deg C for short term storage (1/2 weeks) . Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Shipping Information: *Blue Ice*
Applications: SDS-PAGE
Bioactivity: Specific activity is > 150 nM/min/ug, and is defined as the amount of enzyme that cleaves 1uM of suc-AAFP-pNA per minute at 1 DEG C in Tris-Hcl pH8.0 using chymotrypsin.
Usage Recommendations: N/A
Assay Information: 1. Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1uM suc-AAFP-pNA) . 2. Add recombinant FKBP2 protein with various concentrations (1ug, 2ug, 5ug) in assay buffer. 3. Mix by inversion and equilibrate to 1 DEG C and monitor the A410nm until the value is constant using a spectrophotometer. 4. Add pre-chilled chymotrypsin to 12.5 uM and mix immediately. 5. Record the increase in A410 nm for 20 minutes
