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Product Description
TNF alpha protein | 30R-AT045 | Fitzgerald
Activity Code: ACTIVE
Product Type: Proteins
Product Subtype: Recombinant Proteins
Research Area: Cytokines & Growth Factors
Short Description: Purified recombinant Human TNF alpha protein
Immunogen: N/A
Host: N/A
Specificity: N/A
Cross Reactivity: N/A
Isotype: N/A
IClone: N/A
Species: Human
Residues: Tumor Necrosis Factor-a Human Recombinant protein containing 158 amino acids.
Tag/conjugate: N/A
Protein Type: Recombinant
Expression System: E.coli
Grade & Purity: > 98% pure
Methode of Purification: TNF alpha protein was purified by a proprietary chromatographic technique.
Source: N/A
Concentration: N/A
Form & Buffer: Lyophilized from 20mM PBS, pH 7.2, with 10mM NaCl. Reconstitute in water to a concentration of 0.1 - 1.0mg/ml. Please Note: Buffer may change with new Lots.
Storage: Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Shipping Information: *Blue Ice*
Applications: User optimized
Bioactivity: The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is
Usage Recommendations: N/A
Assay Information: Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 1.234 as the extinction coefficient for a 0.1% (1mg/ml) solution. 2. Analysis by RP-HPLC, using a calibrated solution of TNF-a as a Reference Standard.
