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  • Anti-Vesicular Monoamine Transter VMAT2

    • Anti-Vesicular Monoamine Transter VMAT2 | 240-16085

    NULL340.00

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    SKU:
    240-16085
    Availability:
    IN STOCK
    Size:
    100 µL
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    Product Description

    Anti-Vesicular Monoamine Transter VMAT2 | 240-16085

    biological source

    rabbit

    Quality Level

    100

    antibody form

    affinity isolated antibody

    antibody product type

    primary antibodies

    clone

    polyclonal

    purified by

    affinity chromatography

    species reactivity

    rat

    manufacturer/tradename

    Chemicon®

    technique(s)

    ELISA: suitable
    immunohistochemistry: suitable
    western blot: suitable

    NCBI accession no.

    NM_003054.3

    Specificity

    Recognizes Vesicular Monoamine Transporter 2 (VMAT2). An antibody made to the C-terminal VMAT2 peptide detected a major band at ~55 kDa in postnuclear supernatants of CHO transfected with VMAT2 and not in wild type cells (Peter et al. 1995; Nirenberg et al. 1995). Some additional bands, both higher and lower molecular weight, were also detected and remain unaffected by the inclusion of protease inhibitors. Higher molecular weight bands may represent glycosylated VMAT2 (Peter et al. 1995; Nirenberg et al. 1995, Liu et al. 1994). VMAT2 has been localized in adrenal chromaffin cells, sympathetic ganglion cells, enterochromaffin-like cells of the stomach and in CNS (Peter et al. 1995; Nirenberg et al. 1995; Nirenberg et al. 1996). May recognize human due to the 92% homology between rat and human VMAT2.

    Immunogen

    A 13 amino acid C-terminal peptide sequence from rat VMAT2 (Liu et al. 1992). This peptide is predicted to be cytoplasmic.

    Application

    Research Category
    Neuroscience
    Western blot: 1-10 μg/mL (Chemiluminescence technique)

    Immunohistochemistry: 1-20 μg/mL on paraformaldehyde fixed sections.

    ELISA: 0.5-1.0 μg/mL (1 μg/mL VMAT1 peptide (Catalog Number AG251)/well)

    Optimal working dilutions must be determined by end user.

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    Size 100 µL

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