This antibody is specific for the HA tag containing fusion proteins and does not detect other proteins.
The HA tag (Y-P-Y-D-V-P-D-Y-A) is derived from an epitope (amino acids 98-106) of the influenza hemagglutinin (HA) protein. HA tag is used as a general epitope tag in expression vectors for many recombinant proteins. The presence of HA tag facilitates the detection, isolation and purification of recombinant fusion proteins.
Immunogen
Peptide sequence (Y-P-Y-D-V-P-D-Y-A)HA-Tag.
Application
Rabbit polyclonal anti-HA-Tag antibody may be used as an epitope specific reagent for the detection and/or purification of multiple engineered proteins. The specific advantage is that the use of HA-tag sequence in engineered proteins eliminates the requirement to have a protein specific antibody for detection and purification of specific proteins.
Anti-HA-Tag antibody produced in rabbit has been used in western blotting.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.