Product Description
Liver Tissue Lysate (Tumor) | 1720-01 | ProSci
Tested Application: WB
Application: N/A
Storage Condition: Store at 2-8˚C for continuous use. For extended storage, freeze working aliquots at -70˚C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
User Note:
Background:
Shipping: N/A
Type: Tissue
Physical State: Liquid
Buffer: Liver Tissue Lysate (Tumor) is supplied in SDS sample buffer containing 5% β-mercaptoethanol.
Concentration: 2 mg/mL
Background: Liver tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.
Disclaimer: This product is intended for laboratory research purposes only and should be used by qualified personnel only. ProSci lysates are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci lysates.
Short Description: total protein liver tumor - human
Source: Human Liver Tissue
Tissus By Diagnosis: Diseased
Tissus By Developemental Stage : N/A
Tissus By Species: Human
Tissus/ Cell: Liver
Diagnostic: Tumor
Cancer Type: Trabecular pattern hepatocellular carcinoma
Cancer Type Ezpanded: N/A
Age: 64
Sex: F
Race: Caucasian
Species: Human