Product Description
Iron assay kit (Ferrozine)
Product Description
Iron is a mineral which binds many proteins and enzymes, and plays important roles in living bodies. MC-Reagent Fe Assay Kit provides a simple and convenient tool for the determination of Ferrous and/or Ferric ion in a variety of biological samples such as cell lysates, tissue homogenates, serum, plasma, saliva, urine, tissue extract, plant extract, hair and food extract.
Performance
1. Sensitivity: O.D. at 200 µg/dL standard will be 0.08 - 0.25
2. Accuracy: ±10% of known concentration
3. Intra assay variation: C.V. is less than 5%
4. Assay range: 5 - 1,000 µg/dL
| Specifications |
| Assay principle: |
Ferrozine method (Colorimetric assay at 560nm) |
| Specifity: |
Specific to Fe2+ and Fe3+ |
| Measuring range: |
5 - 1,000 µg/dL |
| Sample volume: |
40 µL |
| Assay conditions: |
10 minutes at room temperature (25 - 37 °C) |
| Applications: |
Serum, plasma, urine, saliva, cell lysate, tissue extract, plant extract, hair extract, food extract and water. Metal chelatos, for example EDTA may interfere with this assay. |
| Storage temperature: |
2 - 8°C (don't freeze). |
| Expiry date: |
1 year after the day of manufacturing. |
| Required but not provided: |
96-well plate
Pipeting devices such as 8-channel (50-200 µL) micropipettor and tips
8 or 12-syncronous multichannel pipet and reagent tray for multichannel pipet.
Microtiter plate reader (measuring wavelength between 540 and 580nm. 560nm is recommended.) |
|
| Content of this kit |
| R-A Buffer (ready to use): |
1 x 40 mL |
| R-R Chelate color (ready to use): |
1 x1.6 mL |
| Iron standard 200 µg/dL (ready to use): |
1 x 8 mL |
|
 |
| References: Assay examples |
| 1) |
Y Ikeda, S Tajima, YI-Ishizawa, Y Kihira, K Ishizawa, S Tomita, K Tsuchiya and T Tamaki:
Estrogen Regulates Hepcidin Expression via GPR30-BMP6-Dependent Signaling in Hepatocytes.
PLoS ONE 7(7),e40465(2012) doi: 10.1371/journal.pone.0040465 |
| 2) |
Tsugawa H, Suzuki H, Matsuzaki J, Hirata K, Hibi T:
FecA1, a bacterial iron transporter, determines the survival of Helicobacter pylori in the stomach.
Free Radical Biology and Medicine 52(6),p1003-1010(2012) doi: 10.1016/j.freeradbiomed.2011.12.011. |
| 3) |
M Nakaya, M Tajima, H Kosako, T Nakaya, A Hashimoto, K Watari, H Nishihara, M Ohba, S Komiya, N Tani, M Nishida, H Taniguchi, Y Sato, M Matsumoto, M Tsuda, M Kuroda, K Inoue and H Kurose:
GRK6 deficiency in mice causes autoimmune disease due to impaired apoptotic cell clearance.
Nature Communications 4, 1532 (2013) doi:10.1038/ncomms2540 |
| 4) |
Y Ikeda, H Enomoto, S Tajima, YI Ishizawa, Y Kihira, K Ishizawa, S Tomita, K Tsuchiya, T Tamaki:
Dietary iron restriction inhibits progression of diabetic nephropathy in db/db mice.
American Journal of Physiology - RenalPhysiology 304:F1028-F1036(2013) DOI:10.1152/ajprenal.00473.2012 |
| 5) |
Y Ikeda, I Ozono, S Tajima, M Imao, Y Horinouchi, YI Ishizawa, Y Kihira, L Miyamoto, K Ishizawa, K Tsuchiya, T Tamaki:
Iron Chelation by Deferoxamine Prevents Renal Interstitial Fibrosis in Mice with Unilateral Ureteral Obstruction.
PLoS ONE 9(2), e89355(2014) doi:10.1371/journal.pone.0089355 |
|
Custom Field
Size 100 Tests
Type of Marker Trace elements assay
Marker Fe
Storage 2 - 8°C (don't freeze).
Colorimetric 740-760 nm
Assay range 10-1,000 ug/dL
Usage Urine, Serum, Tissue
Remark Iron (Fe) is an important pro-oxidant factor which produce hydroxyl radical by fenton reaction. CFE-005 and CFE-010 can be also applied to extractes from animal tissue, plants and hair samples.
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