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Product Description
The ISWE Cortisol Mini-Kit is specifically designed to measure Cortisol present in a variety of samples and species for wildlife conservation. The end user must provide or coat their own anti-rabbit IgG secondary antibody coated plates, in addition to providing assay buffer, wash buffer, substrate and stop solution. See Related Products for suitable options available.
NOTE: The 50X Concentrate Rabbit Antibody to Cortisol cannot be directly coated onto plates for EIA use. It is an unpurified serum diluted in a buffer containing proteins and detergents. The antibody WILL NOT bind to uncoated microtiter plates effectively. The end user must use plates coated with goat or donkey anti-rabbit IgG.
Kits Include:
ISWE Cortisol Antibody Concentrate
ISWE Cortisol Conjugate Concentrate
Cortisol Standard
Storage Instructions
All components of this kit should be aliquoted into vials upon first thaw. Avoid repeat freeze-thaw cycles.
Store at -20°C until the expiration date of the kit.
Protocols
The rabbit anti-Cortisol is supplied at a 50X concentration. An aliquot should be brought to room temperature and diluted one part with 49 parts of a suitable assay buffer. If your assay buffer has protein, detergent and preservative in it, the diluted antibody will be stable for greater than 1 month at 4°C. Please see Related Products for a suitable Assay Buffer.
The Cortisol-HRP conjugate is supplied at a 50X concentration. An aliquot should be brought to room temperature and diluted one part with 49 parts of a suitable diluent or assay buffer. If you wish to store the conjugate diluted for greater than 1 month at 4°C you should dilute it in a suitable conjugate diluent. Please see Related Products for a suitable Conjugate Diluent.
The Cortisol standard stock is provided at 320 ng/mL (320,000 pg/mL) in a special storage solution. The solution contains an organic solvent. Prerinse the pipet tip several times to ensure accurate delivery.
NOTE: The dilution of the ISWE Mini-kit antibody and conjugate are such that when diluted 1:50 in Assay Buffer or Conjugate Diluent and added to an Arbor Assays’ antibody coated plate, using 100 μL of sample or standards, 50 μL of conjugate and 50 μL of antibody in a 2 hour room temperature with shaking incubation, followed by our TMB as substrate, these reagents will generate approximately 1.0 OD at 450 nm. YOUR RESULTS WILL VARY DEPENDING ON A NUMBER OF VARIABLES. You will have to titer the antibody or conjugate to obtain the optimum conditions for your assay system.
