Euro
USD
British Pound
NULL
![Phospho-SMAD2 (S118) Antibody [APR05221G]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/1280x1280/products/867086/1159153/logo__92149.1659788186__82991.1659863492.png?c=2 "Phospho-SMAD2 (S118) Antibody [APR05221G]"){kind=logo}
![Phospho-SMAD2 (S118) Antibody [APR05221G]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/100x100/products/867086/1159153/logo__92149.1659788186__82991.1659863492.png?c=2 "Phospho-SMAD2 (S118) Antibody [APR05221G]"){kind=logo}
Product Description
Phospho-SMAD2 (S118) Antibody [APR05221G] | Leading Biology
Product Category: Polyclonal Antibodies
Host: Rabbit
Species Reactivity: H
Specificity: This SMAD2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S118 of human SMAD2.
Cellular Localisation: Cytoplasm. Nucleus. Note=Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 (PubMed:9865696, PubMed:21145499) . On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1 (PubMed:16751101, PubMed:19289081) . Localized mainly to the nucleus in the early stages of embryo development with expression becoming evident in the cytoplasm at the blastocyst and epiblast stages (By similarity) {ECO:0000250|UniProtKB:Q62432, ECO:0000269|PubMed:16751101, ECO:0000269|PubMed:19289081, ECO:0000269|PubMed:21145499, ECO:0000269|PubMed:9865696}
Molecular Weight: 52306
Clone: Polyclonal
Gene Name: SMAD2
Gene ID: 4087
Function: Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Summary: Tissue Location: Expressed at high levels in skeletal muscle, endothelial cells, heart and placenta.
Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.
Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Application: IF, DB
Dilution: IF--1:10~50 DB--1:500
Synonyms: Mothers against decapentaplegic homolog 2, MAD homolog 2, Mothers against DPP homolog 2, JV18-1, Mad-related protein 2, hMAD-2, SMAD family member 2, SMAD 2, Smad2, hSMAD2, SMAD2, MADH2, MADR2
![Phospho-SMAD2 (S118) Antibody [APR05221G]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/867086/1159153/logo__92149.1659788186__82991.1659863492.png?c=2 "Phospho-SMAD2 (S118) Antibody [APR05221G]"){kind=logo}
![Phospho-Bad (S118) Antibody [APR09133G]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/866869/1158936/logo__92149.1659788186__30690.1659863404.png?c=2 "Phospho-Bad (S118) Antibody [APR09133G]"){kind=logo}
