Euro
USD
British Pound
NULL
![EIF2A Antibody [AMM22217G]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/1280x1280/products/870578/1162645/logo__92149.1659788186__49683.1659866710.png?c=2 "EIF2A Antibody [AMM22217G]"){kind=logo}
![EIF2A Antibody [AMM22217G]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/100x100/products/870578/1162645/logo__92149.1659788186__49683.1659866710.png?c=2 "EIF2A Antibody [AMM22217G]"){kind=logo}
Product Description
EIF2A Antibody [AMM22217G] | Leading Biology
Product Category: Monoclonal Antibodies
Host: Mouse
Species Reactivity: H, M, Rat
Specificity: This EIF2A antibody is generated from a mouse immunized with a recombinant protein of human EIF2A.
Cellular Localisation: Cytoplasm, Stress granule {ECO:0000250|UniProtKB:Q6ZWX6}. Note=Colocalizes with NANOS3 in the stress granules. {ECO:0000250|UniProtKB:Q6ZWX6}
Molecular Weight: 36112
Clone: Monoclonal
Gene Name: EIF2S1 (HGNC:3265)
Gene ID: 1965
Function: Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA (PubMed:16289705) . This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex (PubMed:16289705) . Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex (PubMed:16289705) . In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B (PubMed:16289705) . EIF2S1/eIF-2-alpha is a key component of the integrated stress response (ISR), required for adaptation to various stress: phosphorylation by metabolic-stress sensing protein kinases (EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2) in response to stress converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, leading to an attenuation of cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activators ATF4 and QRICH1, and hence allowing ATF4- and QRICH1-mediated reprogramming (PubMed:19131336, PubMed:33384352) .
Summary: N/A
Form: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Application: WB
Dilution: WB--1:1000
Synonyms: Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, eIF-2-alpha, eIF-2A, eIF-2alpha, EIF2S1, EIF2A
![EIF2A Antibody [AMM22217G]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/870578/1162645/logo__92149.1659788186__49683.1659866710.png?c=2 "EIF2A Antibody [AMM22217G]"){kind=logo}
![EIF2A Antibody [AMM22304G]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/870665/1162732/logo__92149.1659788186__51615.1659866782.png?c=2 "EIF2A Antibody [AMM22304G]"){kind=logo}
