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Product Description
RAB3A Antibody [AMM02525G] | Leading Biology
Product Category: Monoclonal Antibodies
Host: Mouse
Species Reactivity: H, M, Rat
Specificity: This RAB3A antibody is generated from a mouse immunized with a recombinant protein.
Cellular Localisation: Cytoplasm, cytosol {ECO:0000250|UniProtKB:P63012}. Lysosome Cytoplasmic vesicle, secretory vesicle {ECO:0000250|UniProtKB:P63012} Cell projection, axon {ECO:0000250|UniProtKB:P63011}. Cell membrane; Lipid-anchor; Cytoplasmic side. Note=Cycles between a vesicle-associated GTP-bound form and a cytosolic GDP-bound form. {ECO:0000250|UniProtKB:P63012}
Molecular Weight: 24984
Clone: Monoclonal
Gene Name: RAB3A
Gene ID: 5864
Function: Small GTP-binding protein that plays a central role in regulated exocytosis and secretion. Controls the recruitment, tethering and docking of secretory vesicles to the plasma membrane (By similarity) . Upon stimulation, switches to its active GTP-bound form, cycles to vesicles and recruits effectors such as RIMS1, RIMS2, Rabphilin-3A/RPH3A, RPH3AL or SYTL4 to help the docking of vesicules onto the plasma membrane (By similarity) . Upon GTP hydrolysis by GTPase-activating protein, dissociates from the vesicle membrane allowing the exocytosis to proceed (By similarity) . Stimulates insulin secretion through interaction with RIMS2 or RPH3AL effectors in pancreatic beta cells (By similarity) . Regulates calcium-dependent lysosome exocytosis and plasma membrane repair (PMR) via the interaction with 2 effectors, SYTL4 and myosin-9/MYH9 (PubMed:27325790) . Acts as a positive regulator of acrosome content secretion in sperm cells by interacting with RIMS1 (PubMed:22248876, PubMed:30599141) . Plays also a role in the regulation of dopamine release by interacting with synaptotagmin I/SYT (By similarity) . Interacts with MADD (via uDENN domain) ; the GTP-bound form is preferred for interaction (By similarity) .
Summary: Tissue Location: Specifically expressed in brain.
Form: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Application: WB, FC, IF
Dilution: IF--1:25 FC--1:25 WB--1:2000 WB--1:500
Synonyms: Ras-related protein Rab-3A, RAB3A
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