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Product Description
PEF1 Antibody (Center) [APR04204G] | Leading Biology
Product Category: Polyclonal Antibodies
Host: Rabbit
Species Reactivity: H
Specificity: This PEF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-121 amino acids from the Central region of human PEF1.
Cellular Localisation: Cytoplasm. Endoplasmic reticulum {ECO:0000250|UniProtKB:Q641Z8}. Membrane; Peripheral membrane protein. Cytoplasmic vesicle, COPII-coated vesicle membrane; Peripheral membrane protein. Note=Membrane-associated in the presence of Ca (2+) (PubMed:11278427) . Localizes to endoplasmic reticulum exit site (ERES) (By similarity) . {ECO:0000250|UniProtKB:Q641Z8, ECO:0000269|PubMed:11278427}
Molecular Weight: 30381
Clone: Polyclonal
Gene Name: PEF1 (HGNC:30009)
Gene ID: 553115
Function: Calcium-binding protein that acts as an adapter that bridges unrelated proteins or stabilizes weak protein-protein complexes in response to calcium. Together with PDCD6, acts as calcium-dependent adapter for the BCR (KLHL12) complex, a complex involved in endoplasmic reticulum (ER) -Golgi transport by regulating the size of COPII coats (PubMed:27716508) . In response to cytosolic calcium increase, the heterodimer formed with PDCD6 interacts with, and bridges together the BCR (KLHL12) complex and SEC31 (SEC31A or SEC31B), promoting monoubiquitination of SEC31 and subsequent collagen export, which is required for neural crest specification (PubMed:27716508) . Its role in the heterodimer formed with PDCD6 is however unclear: some evidence shows that PEF1 and PDCD6 work together and promote association between PDCD6 and SEC31 in presence of calcium (PubMed:27716508) . Other reports show that PEF1 dissociates from PDCD6 in presence of calcium, and may act as a negative regulator of PDCD6 (PubMed:11278427) . Also acts as a negative regulator of ER-Golgi transport; possibly by inhibiting interaction between PDCD6 and SEC31 (By similarity) .
Summary: N/A
Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Application: WB
Dilution: WB--1:1000
Synonyms: Peflin, PEF protein with a long N-terminal hydrophobic domain, Penta-EF hand domain-containing protein 1, PEF1, ABP32
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