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Product Description
PIWIL2 Antibody (N-term) [AMM07206G] | Leading Biology
Product Category: Polyclonal Antibodies
Host: Rabbit
Species Reactivity: H
Specificity: This PIWIL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 90-119 amino acids from the N-terminal region of human PIWIL2.
Cellular Localisation: Cytoplasm {ECO:0000250|UniProtKB:Q8CDG1}. Note=Present in chromatoid body. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis {ECO:0000250|UniProtKB:Q8CDG1}
Molecular Weight: 109849
Clone: Polyclonal
Gene Name: PIWIL2
Gene ID: 55124
Function: Endoribonuclease that plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity (By similarity) . Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells (By similarity) . Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons (By similarity) . During piRNA biosynthesis, plays a key role in the piRNA amplification loop, also named ping-pong amplification cycle, by acting as a 'slicer-competent' piRNA endoribonuclease that cleaves primary piRNAs, which are then loaded onto 'slicer-incompetent' PIWIL4 (By similarity) . PIWIL2 slicing produces a pre-miRNA intermediate, which is then processed in mature piRNAs, and as well as a 16 nucleotide by- product that is degraded (By similarity) . Required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense (By similarity) . Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation (By similarity) . Indirectly modulates expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8 (By similarity) . When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors (PubMed:16377660) . Represses circadian rhythms by promoting the stability and activity of core clock components ARNTL/BMAL1 and CLOCK by inhibiting GSK3B-mediated phosphorylation and ubiquitination- dependent degradation of these proteins (PubMed:28903391) .
Summary: Tissue Location: Expressed in adult testis and in most tumors.
Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Application: WB
Dilution: WB--1:500 WB--1:1000
Synonyms: Piwi-like protein 2, Cancer/testis antigen 80, CT80, PIWIL2, HILI
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