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Product Description
LIG4 Antibody (N-term) [APR32783G] | Leading Biology
Product Category: Polyclonal Antibodies
Host: Rabbit
Species Reactivity: H
Specificity: This LIG4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 239-267 amino acids from the N-terminal region of human LIG4.
Cellular Localisation: Nucleus
Molecular Weight: 103971
Clone: Polyclonal
Gene Name: LIG4 {ECO:0000303|PubMed:16357942, ECO:0000312|HGNC:HGNC:6601}
Gene ID: 3981
Function: DNA ligase involved in DNA non-homologous end joining (NHEJ) ; required for double-strand break (DSB) repair and V (D) J recombination (PubMed:8798671, PubMed:9242410, PubMed:9809069, PubMed:12517771, PubMed:17290226) . Catalyzes the NHEJ ligation step of the broken DNA during DSB repair by resealing the DNA breaks after the gap filling is completed (PubMed:9242410, PubMed:9809069, PubMed:12517771, PubMed:17290226) . Joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction (PubMed:9242410, PubMed:9809069, PubMed:12517771, PubMed:17290226) . LIG4 is mechanistically flexible: it can ligate nicks as well as compatible DNA overhangs alone, while in the presence of XRCC4, it can ligate ends with 2-nucleotides (nt) microhomology and 1-nt gaps (PubMed:17290226) . Forms a subcomplex with XRCC4; the LIG4-XRCC4 subcomplex is responsible for the NHEJ ligation step and XRCC4 enhances the joining activity of LIG4 (PubMed:9242410, PubMed:9809069) . Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends (PubMed:10854421) . LIG4 regulates nuclear localization of XRCC4 (PubMed:24984242) .
Summary: Tissue Location: Testis, thymus, prostate and heart.
Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Application: WB
Dilution: WB--1:1000
Synonyms: DNA ligase 4, DNA ligase IV, Polydeoxyribonucleotide synthase [ATP] 4, LIG4
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