Euro
USD
British Pound
NULL
![COT (MAP3K8/MEKK8) Antibody (C-term) [APR06031G]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/1280x1280/products/868397/1160464/logo__92149.1659788186__18056.1659864361.png?c=2 "COT (MAP3K8/MEKK8) Antibody (C-term) [APR06031G]"){kind=logo}
![COT (MAP3K8/MEKK8) Antibody (C-term) [APR06031G]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/100x100/products/868397/1160464/logo__92149.1659788186__18056.1659864361.png?c=2 "COT (MAP3K8/MEKK8) Antibody (C-term) [APR06031G]"){kind=logo}
Product Description
COT (MAP3K8/MEKK8) Antibody (C-term) [APR06031G] | Leading Biology
Product Category: Polyclonal Antibodies
Host: Rabbit
Species Reactivity: H
Specificity: This COT (MAP3K8/MEKK8) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 414-445 amino acids from the C-terminal region of human COT (MAP3K8/MEKK8) .
Cellular Localisation: Cytoplasm
Molecular Weight: 52925
Clone: Polyclonal
Gene Name: MAP3K8
Gene ID: 1326
Function: Required for lipopolysaccharide (LPS) -induced, TLR4-mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the proinflammatory cytokine TNF-alpha (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. In vitro, activates MAPK/ERK pathway in response to IL1 in an IRAK1-independent manner, leading to up-regulation of IL8 and CCL4. Transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB- dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle. Isoform 1 shows some transforming activity, although it is much weaker than that of the activated oncogenic variant.
Summary: Tissue Location: Expressed in several normal tissues and human tumor-derived cell lines
Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Application: WB, IHC-P
Dilution: WB--1:1000 IHC-P--1:100~500
Synonyms: Mitogen-activated protein kinase kinase kinase 8, Cancer Osaka thyroid oncogene, Proto-oncogene c-Cot, Serine/threonine-protein kinase cot, Tumor progression locus 2, TPL-2, MAP3K8, COT, ESTF
![COT (MAP3K8/MEKK8) Antibody (C-term) [APR06031G]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/868397/1160464/logo__92149.1659788186__18056.1659864361.png?c=2 "COT (MAP3K8/MEKK8) Antibody (C-term) [APR06031G]"){kind=logo}
