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Product Description
CYP11A1 Antibody (N-term) [APG02910G] | Leading Biology
Product Category: Polyclonal Antibodies
Host: Rabbit
Species Reactivity: H
Specificity: This CYP11A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88-120 amino acids from the N-terminal region of human CYP11A1.
Cellular Localisation: Mitochondrion inner membrane {ECO:0000250|UniProtKB:P14137}; Peripheral membrane protein. Note=Localizes to the matrix side of the mitochondrion inner membrane. {ECO:0000250|UniProtKB:P14137}
Molecular Weight: 60102
Clone: Polyclonal
Gene Name: CYP11A1 {ECO:0000303|PubMed:21636783, ECO:0000312|HGNC:HGNC:2590}
Gene ID: 1583
Function: A cytochrome P450 monooxygenase that catalyzes the side-chain hydroxylation and cleavage of cholesterol to pregnenolone, the precursor of most steroid hormones (PubMed:21636783) . Catalyzes three sequential oxidation reactions of cholesterol, namely the hydroxylation at C22 followed with the hydroxylation at C20 to yield 20R, 22R- hydroxycholesterol that is further cleaved between C20 and C22 to yield the C21-steroid pregnenolone and 4-methylpentanal (PubMed:21636783) . Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin) (PubMed:21636783) .
Summary: N/A
Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Application: WB
Dilution: WB--1:1000
Synonyms: Cholesterol side-chain cleavage enzyme, mitochondrial, CYPXIA1, Cholesterol desmolase, Cytochrome P450 11A1, Cytochrome P450 (scc), CYP11A1, CYP11A
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