Product Description
EHHADH Antibody (C-term) [APR07649G] | Leading Biology
Product Category: Polyclonal Antibodies
Host: Rabbit
Species Reactivity: H, M
Specificity: This EHHADH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 662-690 amino acids from the C-terminal region of human EHHADH.
Cellular Localisation: Peroxisome.
Molecular Weight: 79495
Clone: Polyclonal
Gene Name: EHHADH (HGNC:3247)
Gene ID: 1962
Function: Peroxisomal trifunctional enzyme possessing 2-enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase, and delta 3, delta 2-enoyl- CoA isomerase activities. Catalyzes two of the four reactions of the long straight chain fatty acids peroxisomal beta-oxidation pathway. Optimal isomerase for 2, 5 double bonds into 3, 5 form isomerization in a range of enoyl-CoA species (Probable) . Also able to isomerize both 3- cis and 3-trans double bonds into the 2-trans form in a range of enoyl- CoA species (By similarity) . With HSD17B4, catalyzes the hydration of trans-2-enoyl-CoA and the dehydrogenation of 3-hydroxyacyl-CoA, but with opposite chiral specificity (PubMed:15060085) . Regulates the amount of medium-chain dicarboxylic fatty acids which are essential regulators of all fatty acid oxidation pathways (By similarity) . Also involved in the degradation of long-chain dicarboxylic acids through peroxisomal beta-oxidation (PubMed:15060085) .
Summary: Tissue Location: Liver and kidney. Strongly expressed in the terminal segments of the proximal tubule. Lower amounts seen in the brain.
Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Application: WB, IHC-P, FC
Dilution: WB--1:1000 IHC-P--1:100~500 FC--1:10~50
Synonyms: Peroxisomal bifunctional enzyme, PBE, PBFE, Enoyl-CoA hydratase/3, 2-trans-enoyl-CoA isomerase, 3-hydroxyacyl-CoA dehydrogenase, EHHADH, ECHD