Product Description
Magnesium assay kit (Xyridil Blue-I)
[ Product Description ]
Magnesium is one of the most abundant minerals in the living cells, and plays important roles in many metabolic reactions such as polyphosphoric acid formation and photosynthesis in the chlorophyll.
[ Assay principle ]
Under alkaline condition, Xyridyl Blue-I(XB-I) - magnesium complex shows absorbance at 520 nm, and on the other hand, the absorbance by free XB-I at 600-680nm decreases.
[ Performance ]
1. Sensitivity: 2. Accuracy: 3. Intra assay variation: 4. Assay range: |
O.D. at 2 mg/dL standard will be 0.05 - 0.1(660nm vs 700nm) ±15% of known concentration C.V. is less than 5% 0.05 - 3.5 mg/dL |

[ Specifications ] |
Assay principle: |
XB-I method (Colorimetric assay at 520-660 vs 700 nm) Measuring wavelength: 660nm (reference 700 nm) is recommended. Measuring at 520 nm vs 700 nm shows higher sensitivity but may be susceptible to various factors. |
Specifity: |
Magnesium (Mg) |
Measuring range: |
0.05 - 3.5 mg/dL |
Assay conditions: |
5 minutes at room temperature (25 - 37 °C) |
Applications: |
Serum, urine, saliva, cell lysate, tissue extract, plant extract, hair extract, food extract and water. This assay will be interfered by EDTA, hemoglobin, anticoagulants and high concentration of potassium. |
Storage temperature: |
2 - 8°C |
Expiry date: |
1 year after the day of manufacturing. |
Required but not provided: |
Distilled water 96 well microtiter plate Micropipettors and pipette tips Microtiter plate reader (measuring wavelength 660/700 nm) |
|
[ Contents of this kit ] |
R-1 Chelate color (ready to use): |
25 mL |
Magnesium standard 2 mg/dL (ready to use): |
0.5 mL |
|
[ References: Assay examples ] |
1) |
Akanuma G, Kobayashi A, Suzuki S, Kawamura F, Shiwa Y, Watanabe S, Yoshikawa H, Hanai R, Ishizuka M: Defect in the formation of 70S ribosomes caused by lack of ribosomal protein L34 can be suppressed by magnesium. J Bacteriol 196(22),p3820-3830(2014) doi: 10.1128/JB.01896-14 |
2) |
Sakamoto A, Terui Y, Yamamoto T, Kasahara T, Nakamura M, Tomitori H, Yamamoto K, Ishihama A, Michael AJ, Igarashi K, Kashiwagi K: Enhanced biofilm formation and/or cell viability by polyamines through stimulation of response regulators UvrY and CpxR in the two-component signal transducing systems, and ribosome recycling factor. Int J Biochem Cell Biol 44(11),p1877-1886(2012). doi: 10.1016/j.biocel.2012.07.010 |
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Size
Size 100 Tests
Type of Marker Trace elements assay
Marker Mg
Storage 2 - 8°C.
Colorimetric 660 nm
Assay range 0.05-3.5 mg/dL
Usage Urine, Serum, Tissue
Remark Applicable to serum, plasma, urine, saliva, extracts from tissues, plants and hair. May be interfared by EDTA, haemolysis, anticoagulants and potassium.