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Product Description
This product is a 8-OHdG ELISA kit utilizing anti 8-OHdG monoclonal antibody (clone N45.1) which is highly specific for 8-OHdG. We provide two types of 8-OHdG ELISA kits with different assay range. New 8-OHdG Check ELISA is suitable for urine and serum sample from animal and human. If you are planning to measure 8-OHdG in human serum,tissue, cultured cells, we recommend to use 'Highly Sensitive 8-OHdG Check ELISA'.
Specifications
Assay principle: Competitive ELISA (detection: 450 nm)
Specifity: Specific for 8-OHdG. Antibody have been tested to 8-OHdG analogues (guanosine(G),7-methyl-G, 6-SH-G, 8-Bromo-G, dA, dC, dT, dI, dU, dG, O6-methyl-dG,8-OHdA, guanine(Gua),O6-methyl-Gua, 8-OH-Gua, uric acid, urea, creatine, creatinine, 8-sulfhydryl-G, 8-OH-G).
Measuring range: 0.5 to 200 ng/mL
Format: 96 wells (18 samples in triple assays)
Applications:
- Urine from human and animals.
- For serum and tissue samples, Highly Sensitive 8-OHdG Check (code #KOG-HS10E) is recommended.
Storage:
- Store at 4 - 10°C (don't freeze).
- 12 months after the day of manufacturing.
Required but not provided:
- Micropipet and chip (100 micro L, 1000 micro L).
- Measuring pipet (10 mL, 20 mL)/ measuring cylinders.
- 8 or 12-syncronous multichannel pipet and reagent tray for multichannel pipet.
- Microplate reader (filter; 450 nm).
Content of this kit
| 8-OHdG Microtiter Plate: | Precoated with 8-OHdG(12 X 8wells, split type) | 1 plate |
| Primary Antibody: | Anti 8-OHdG antibody, powder. | 1 vial |
| Primary Antibody Solution | 1 vial (6mL) | |
| Secondary Antibody: | HRP-anti mouse antibody, powder. | 1 vial |
| Secondary Antibody Solution: | 1 vial (12mL) | |
| Chromatic Solution: | 3,3',5,5'-tetramethylbenzidine | 1 vial (0.25mL) |
| Diluting Solution: | H2O2 Containing Buffer | 1 vial (12mL) |
| Washing Solution(5x): | 2 vials (26mL x 2) | |
| Reaction Terminating Solution: | 1M Phosphoric acid. | 1 vial (12mL) |
| Standard 8-OHdG Solution: | Purified 8-OHdG (0.5, 2, 8, 20, 80, 200 ng/mL) | 1 vial each |
| Plate Seal: | 2 sheets |
