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Product Description
CD80 Detection Set (Risk Free) | RF16040 | ProSci
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: CD80 antibodies were raised against the extracellular domain of human CD80.
Tested Application: E, WB, ICC, IHC-P, Flow, IF
Application: N/A
Specificity: N/A
Purification: Antibodies are supplied as protein A purified IgG1 and IgG2b.
Clonality: Monoclonal
Isotype: IgG1, IgG2b
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS containing 0.02% sodium azide.
Concentration: Antibody 1 mg/mL
Storage Condition: Antibodies can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Background: CD80, also known as B7-1, is a type I membrane protein that is a member of the immunoglobulin superfamily. Like the related protein CD86, this protein is expressed by antigen-presenting cells, and is the ligand for two proteins at the cell surface of T cells, CD28 and the cytotoxic T-lymphocyte-associated protein 4 (CTLA-4). Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell and induces T-cell proliferation and cytokine production. CTLA-4 binding negatively regulates T-cell activation and diminishes the immune response (1). Blocking the CTLA-4-CD80/CD86 interaction has been shown to enhance T-cell functions in acute lymphoblastomic leukemia (ALL), suggesting that this pathway may be an attractive target for future cancer immunotherapy (2). ProSci's Risk-FreeTM antibodies are mouse monoclonal antibodies made to improve in vivo studies. Unlike antibodies developed using proteins made in yeast or bacteria, Risk-FreeTM antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. Validated for flow cytometry and ELISA Rigorously tested for the following applications: Immunoblot Immunohistochemistry Immunocytochemistry Immunofluorescence Multiple antibodies per target allowing the user to choose the best antibody for their application Available individually or as a set Risk-FreeTM means they are guaranteed to work for their approved applications
User Note: Optimal dilutions for each application to be determined by the researcher.
