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Product Description
TIM-3 Detection Set (Risk Free) | RF16100 | ProSci
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: TIM-3 antibody was raised against the extracellular domain of human TIM-3
Tested Application: E, WB, ICC, IHC-P, Flow, IF
Application: N/A
Specificity: N/A
Purification: Antibodies are supplied as protein A purified IgG1.
Clonality: Monoclonal
Isotype: IgG1, IgG2b
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS containing 0.02% sodium azide.
Concentration: Antibody 1 mg/mL
Storage Condition: Antibodies can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Background: The immune checkpoint protein TIM3 is a member of the immunoglobulin superfamily and TIM family of proteins that was initially identified as a specific marker of fully differentiated IFN-γ producing CD4 T helper 1 (Th1) and CD8 cytotoxic cells. It is a Th1-specific cell surface protein that regulates macrophage activation and negatively regulates Th1-mediated auto- and alloimmune responses, and is also highly expressed on regulatory T cells, monocytes, macrophages, and dendritic cells (1). TIM3 and PD-1 are co-expressed on most CD4 and CD8 T cells infiltrating solid tumors or in hematologic malignancy in mice; blocking TIM3 in conjugation with a PD-1 blockade increases the functionality of exhausted T cells and synergizes with to inhibit tumor growth (2,3). ProSci's Risk-FreeTM antibodies are mouse monoclonal antibodies made to improve in vivo studies. Unlike antibodies developed using proteins made in yeast or bacteria, Risk-FreeTM antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. Validated for flow cytometry and ELISA Rigorously tested for the following applications: Immunoblot Immunohistochemistry Immunocytochemistry Immunofluorescence Multiple antibodies per target allowing the user to choose the best antibody for their application Available individually or as a set Risk-FreeTM means they are guaranteed to work for their approved applications
User Note: Optimal dilutions for each application to be determined by the researcher.
